CD14 Antibody

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NSJ Bioreagents
Product Code: NSJ-F49568
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49568-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49568-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the CD14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of CD14 antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
2 / 5
Western blot analysis of CD14 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (2).
3 / 5
IHC analysis of FFPE human lung carcinoma tissue stained with CD14 antibody
4 / 5
CD14 antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
5 / 5
Western blot testing of 1) human liver and 2) human lung lysate with CD14 antibody at 1:2000 dilution. Expected molecular weight: 40-55 kDa depending on glycosylation level.
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Confocal immunofluorescent analysis of CD14 antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Western blot analysis of CD14 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (2).
IHC analysis of FFPE human lung carcinoma tissue stained with CD14 antibody
CD14 antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot testing of 1) human liver and 2) human lung lysate with CD14 antibody at 1:2000 dilution. Expected molecular weight: 40-55 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 54-83 from the human protein was used as the immunogen for this CD14 antibody.
Limitation:
This CD14 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P08571