LIN28B Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F44237
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44237-0.05ML0.05 ml£260.00
Quantity:
NSJ-F44237-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the LIN28B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot analysis of LIN28B antibody and 1) HeLa, 2) K562, 3) mouse testis and 4) NCCIT lysate.
2 / 6
IHC analysis of FFPE human testis tissue stained with LIN28B antibody
3 / 6
IHC analysis of FFPE human spleen tissue stained with LIN28B antibody
4 / 6
Flow cytometric analysis of HL-60 cells using LIN28B antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
5 / 6
Confocal immunofluorescent analysis of LIN28B antibody and HepG2 cells with Alexa Fluor 488-conjugated secondary (green). DAPI was used to stain the cell nuclear (blue).
6 / 6
IHC analysis of FFPE human placental tissue stained with LIN28B antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.
❮❯

Western blot analysis of LIN28B antibody and 1) HeLa, 2) K562, 3) mouse testis and 4) NCCIT lysate.
IHC analysis of FFPE human testis tissue stained with LIN28B antibody
IHC analysis of FFPE human spleen tissue stained with LIN28B antibody
Flow cytometric analysis of HL-60 cells using LIN28B antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of LIN28B antibody and HepG2 cells with Alexa Fluor 488-conjugated secondary (green). DAPI was used to stain the cell nuclear (blue).
IHC analysis of FFPE human placental tissue stained with LIN28B antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
LIN28B is a suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 95-128 from the human protein was used as the immunogen for this LIN28B antibody.
Limitation:
This LIN28B antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
Q6ZN17