MICA Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F51246
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51246-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51246-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the MICA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 7
Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: Daudi ; 3: A431; 4: MCF-7; 5: U-87MG; 6: THP-1; Predicted molecular weight: 38-62 kDa depending on glycosylation level.
2 / 7
Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: U-87 MG lysate; 2: A431; 3: Jurkat; Predicted molecular weight: 38-62 kDa depending on glycosylation level.
3 / 7
Western blot testing of MICA antibody at 1:1000 dilution + HeLa lysate
4 / 7
Flow cytometric analysis of SK-BR-3 cells using MICA antibody (green) compared to an isotype control of <a href=../search_result.php?search_txt=N1001>rabbit IgG (blue)</a>. Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary.
5 / 7
Immunofluorescent staining of fixed and permeabilized human HeLa cells with MICA antibody (green), phalloidin (red, cytoplasmic actin) and DAPI (blue, nuclear).
6 / 7
Immunofluorescent staining of fixed and permeabilized human HeLa cells with MICA antibody (green), phalloidin (red, cytoplasmic actin) and DAPI (blue, nuclear).
7 / 7
IHC staining of FFPE human skin with MICA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
❮❯

Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: Daudi ; 3: A431; 4: MCF-7; 5: U-87MG; 6: THP-1; Predicted molecular weight: 38-62 kDa depending on glycosylation level.
Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: U-87 MG lysate; 2: A431; 3: Jurkat; Predicted molecular weight: 38-62 kDa depending on glycosylation level.
Western blot testing of MICA antibody at 1:1000 dilution + HeLa lysate
Flow cytometric analysis of SK-BR-3 cells using MICA antibody (green) compared to an isotype control of <a href=../search_result.php?search_txt=N1001>rabbit IgG (blue)</a>. Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with MICA antibody (green), phalloidin (red, cytoplasmic actin) and DAPI (blue, nuclear).
Immunofluorescent staining of fixed and permeabilized human HeLa cells with MICA antibody (green), phalloidin (red, cytoplasmic actin) and DAPI (blue, nuclear).
IHC staining of FFPE human skin with MICA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:1000,Flow cytometry: 1:25,Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25
Application Note:
Titration of the MICA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 68-97 from the human protein was used as the immunogen for this MICA antibody.
Limitation:
This MICA antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q29983