Nestin Antibody

NSJ Bioreagents
Product Code: NSJ-F47834
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F47834-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47834-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Nestin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of Nestin antibody at 1:2000 dilution + HeLa lysate; Predicted band size : 170-220 kDa.
2 / 5
Fluorescent confocal image of SY5Y cells stained with Nestin antibody at 1:200. Note the highly specific localization of the Nestin immunosignal to the intermediate filaments.
3 / 5
Western blot analysis of Nestin antibody and HepG2 lysate
4 / 5
IHC analysis of FFPE human hepatocarcinoma stained with Nestin antibody
5 / 5
Flow cytometric analysis of HepG2 cells using Nestin antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot testing of Nestin antibody at 1:2000 dilution + HeLa lysate; Predicted band size : 170-220 kDa.
Fluorescent confocal image of SY5Y cells stained with Nestin antibody at 1:200. Note the highly specific localization of the Nestin immunosignal to the intermediate filaments.
Western blot analysis of Nestin antibody and HepG2 lysate
IHC analysis of FFPE human hepatocarcinoma stained with Nestin antibody
Flow cytometric analysis of HepG2 cells using Nestin antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the Nestin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1389-1416 from the human protein was used as the immunogen for this Nestin antibody.
Limitation:
This Nestin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P48681