PML Antibody

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NSJ Bioreagents
Product Code: NSJ-F48066
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48066-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48066-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PML antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of PML antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
2 / 4
PML antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 4
Western blot analysis of PML antibody and HeLa lysate. There are multiple isoforms from 47-90 kDa.
4 / 4
IHC analysis of FFPE human hepatocarcinoma tissue stained with the PML antibody
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Confocal immunofluorescent analysis of PML antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
PML antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of PML antibody and HeLa lysate. There are multiple isoforms from 47-90 kDa.
IHC analysis of FFPE human hepatocarcinoma tissue stained with the PML antibody

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PML antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL).
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 470-499 (sumoylation site) from the human protein was used as the immunogen for this PML antibody.
Limitation:
This PML antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P29590