Rab5 Antibody

NSJ Bioreagents
Product Code: NSJ-F49994
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49994-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49994-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Rab5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Rab5 antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
2 / 4
IHC analysis of FFPE human brain tissue with Rab5 antibody
3 / 4
Immunofluorescence analysis of Rab5 antibody with paraffin-embedded human brain tissue. Primary antibody was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. Red counterstaining is PI.
4 / 4
Western blot analysis of Rab5 antibody and HL-60 lysate.

Rab5 antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human brain tissue with Rab5 antibody
Immunofluorescence analysis of Rab5 antibody with paraffin-embedded human brain tissue. Primary antibody was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. Red counterstaining is PI.
Western blot analysis of Rab5 antibody and HL-60 lysate.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the Rab5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Rab5 is required for the fusion of plasma membranes and early endosomes.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 182-211 from human Rab5a was used as the immunogen for this Rab5 antibody.
Limitation:
This Rab5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P20339