RUNX1 Antibody

NSJ Bioreagents
Product Code: NSJ-F48224
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48224-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48224-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the RUNX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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RUNX1 antibody western blot analysis in U-937 lysate.
2 / 4
IHC analysis of FFPE human brain tissue stained with RUNX1 antibody
3 / 4
Confocal immunofluorescent analysis of RUNX1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
4 / 4
RUNX1 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.

RUNX1 antibody western blot analysis in U-937 lysate.
IHC analysis of FFPE human brain tissue stained with RUNX1 antibody
Confocal immunofluorescent analysis of RUNX1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
RUNX1 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the RUNX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The RUNX1 protein represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 227-255 from the human protein was used as the immunogen for this RUNX1 antibody.
Limitation:
This RUNX1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q01196