Alkaline Phosphatase Antibody (intestinal/placental)

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NSJ Bioreagents
Product Code: NSJ-F44184
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44184-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44184-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the anti-Alkaline Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from human placenta tissue lysate using anti-Alkaline Phosphatase antibody diluted at 1:1000.
2 / 3
Immunohistochemical analysis of paraffin-embedded human small intestine section using anti-Alkaline Phosphatase antibody diluted at 1:100 dilution.
3 / 3
Fluorescent confocal image of HeLa cells stained with anti-Alkaline Phosphatase antibody at 1:100. Immunoreactivity is localized to the cytoplasm.
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Western blot analysis of lysate from human placenta tissue lysate using anti-Alkaline Phosphatase antibody diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded human small intestine section using anti-Alkaline Phosphatase antibody diluted at 1:100 dilution.
Fluorescent confocal image of HeLa cells stained with anti-Alkaline Phosphatase antibody at 1:100. Immunoreactivity is localized to the cytoplasm.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-100,Immunofluorescence: 1:100
Application Note:
Titration of the anti-Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 274-304 from human ALPI was used as the immunogen for this anti-Alkaline Phosphatase antibody. This sequence is 87% homologous in human ALPP.
Limitation:
This anti-Alkaline Phosphatase antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P09923