CUX1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F49389
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49389-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49389-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the CUX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 7
Fluorescent confocal image of human HeLa cell stained with CUX1 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). Green = CUX1 antibody, Red = Phalloidin stained actin.
2 / 7
Fluorescent staining of human MCF-7 cells with CUX1 antibody. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). Green = CUX1 antibody, Red = Phalloidin stained actin.
3 / 7
IHC staining of FFPE human kidney with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human kidney with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human lymph node with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 7
Western blot testing of human 1) MCF-7, 2) MOLT-4 and 3) SH-SY5Y cell lysate with CUX1 antibody at 1:2000. Expected molecular weight ~200 kDa with smaller isoforms.
7 / 7
Western blot testing of human 1) HEK293 and 2) K562 cell lysate with CUX1 antibody at 1:2000. Expected molecular weight ~200 kDa with smaller isoforms.
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Fluorescent confocal image of human HeLa cell stained with CUX1 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). Green = CUX1 antibody, Red = Phalloidin stained actin.
Fluorescent staining of human MCF-7 cells with CUX1 antibody. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). Green = CUX1 antibody, Red = Phalloidin stained actin.
IHC staining of FFPE human kidney with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human kidney with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lymph node with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 1) MCF-7, 2) MOLT-4 and 3) SH-SY5Y cell lysate with CUX1 antibody at 1:2000. Expected molecular weight ~200 kDa with smaller isoforms.
Western blot testing of human 1) HEK293 and 2) K562 cell lysate with CUX1 antibody at 1:2000. Expected molecular weight ~200 kDa with smaller isoforms.

Documents

Further Information

Application Details :
Western blot: 1:1000-1:2000,IHC (Paraffin): 1:25-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the CUX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CUX1 likely has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity). [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1347-1374 from the human protein was used as the immunogen for this CUX1 antibody.
Limitation:
This CUX1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P39880