HRAS Antibody

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NSJ Bioreagents
Product Code: NSJ-F50750
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50750-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50750-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the HRAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from 293T cell line using HRAS antibody diluted at 1:1000. Predicted molecular weight ~21 kDa.
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Western blot analysis of HRAS antibody in Jurkat lysate. Predicted molecular weight ~21 kDa.
3 / 4
IHC analysis of FFPE human lung carcinoma tissue stained with HRAS antibody
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Confocal immunofluorescent analysis of HRAS antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of lysate from 293T cell line using HRAS antibody diluted at 1:1000. Predicted molecular weight ~21 kDa.
Western blot analysis of HRAS antibody in Jurkat lysate. Predicted molecular weight ~21 kDa.
IHC analysis of FFPE human lung carcinoma tissue stained with HRAS antibody
Confocal immunofluorescent analysis of HRAS antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:500-1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the HRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. These proteins function in signal transduction pathways. They can bind GTP and GDP, and they have intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in the HRAS gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 104-128 from the human protein was used as the immunogen for this HRAS antibody.
Limitation:
This HRAS antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
P01112