mTOR Antibody (FRAP1)

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NSJ Bioreagents
Product Code: NSJ-F49548
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49548-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49548-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the mTOR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of mTOR antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the mTOR gene (2).
2 / 3
IHC analysis of FFPE human testis tissue stained with mTOR antibody
3 / 3
Confocal immunofluorescent analysis of mTOR antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of mTOR antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the mTOR gene (2).
IHC analysis of FFPE human testis tissue stained with mTOR antibody
Confocal immunofluorescent analysis of mTOR antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the mTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FRAP1/mTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. It is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 2459-2488 from the human protein was used as the immunogen for this mTOR antibody.
Limitation:
This mTOR antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
P42345