Osteopontin Antibody

NSJ Bioreagents
Product Code: NSJ-F41543
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41543-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41543-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Osteopontin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of Osteopontin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the OPN-a/b gene.
2 / 3
Osteopontin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue.
3 / 3
Confocal immunofluorescent analysis of Osteopontin antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Western blot analysis of Osteopontin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the OPN-a/b gene.
Osteopontin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue.
Confocal immunofluorescent analysis of Osteopontin antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the Osteopontin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 14-40 from the human protein was used as the immunogen for this Osteopontin antibody.
Limitation:
This Osteopontin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P10451