Phospho-ERBB4 Antibody (pY1162)

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NSJ Bioreagents
Product Code: NSJ-F48403
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48403-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48403-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the phospho-ERBB4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot testing of phospho-ERBB4 antibody and FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min. Ab was used at 1:750. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
2 / 3
Confocal immunofluorescent analysis of phospho-ERBB4 antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
3 / 3
IHC analysis of FFPE human hepatocarcinoma tissue stained with the phospho-ERBB4 antibody.
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Western blot testing of phospho-ERBB4 antibody and FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min. Ab was used at 1:750. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Confocal immunofluorescent analysis of phospho-ERBB4 antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human hepatocarcinoma tissue stained with the phospho-ERBB4 antibody.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the phospho-ERBB4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HER4/ERBB4 is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2 and ERBB3. It is a receptor for NDF/heregulin.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-ERBB4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1162 of human ERBB4.
Limitation:
This phospho-ERBB4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
Q15303