TBP Antibody

NSJ Bioreagents
Product Code: NSJ-F49783
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49783-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49783-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the TBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of TBP antibody and HL-60 lysate. Observed molecular weight: 35-43 kDa.
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IHC analysis of FFPE human testis tissue stained with TBP antibody
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Confocal immunofluorescent analysis of TBP antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Western blot analysis of TBP antibody and HL-60 lysate. Observed molecular weight: 35-43 kDa.
IHC analysis of FFPE human testis tissue stained with TBP antibody
Confocal immunofluorescent analysis of TBP antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 301-330 from the human protein was used as the immunogen for this TBP antibody.
Limitation:
This TBP antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Chicken, Mouse, Primate, Xenopus
Uniprot #:
P20226