Estrogen Receptor Antibody

NSJ Bioreagents
Product Code: NSJ-F43055
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F43055-0.08ML0.08 ml£260.00
Quantity:
NSJ-F43055-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the Estrogen Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Estrogen Receptor antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma.
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Estrogen Receptor antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue.
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Estrogen Receptor antibody western blot analysis in MCF-7 lysate. Predicted molecular weight: 65-70 kDa.
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Confocal immunofluorescent analysis of Estrogen Receptor antibody with ZR-75-1 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Estrogen Receptor antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma.
Estrogen Receptor antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue.
Estrogen Receptor antibody western blot analysis in MCF-7 lysate. Predicted molecular weight: 65-70 kDa.
Confocal immunofluorescent analysis of Estrogen Receptor antibody with ZR-75-1 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Estrogen Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes an Estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. ERs are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 246-273 from the human protein (alpha) was used as the immunogen for this Estrogen Receptor antibody.
Limitation:
This Estrogen Receptor antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Pig
Uniprot #:
P03372