AKT2 Antibody

NSJ Bioreagents
Product Code: NSJ-F50041
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50041-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50041-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the AKT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot analysis of AKT2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).
2 / 4
Fluorescent confocal image of C2C12 cells stained with AKT2 antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
3 / 4
IHC analysis of FFPE human lung carcinoma tissue stained with AKT2 antibody
4 / 4
AKT2 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of AKT2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).
Fluorescent confocal image of C2C12 cells stained with AKT2 antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
IHC analysis of FFPE human lung carcinoma tissue stained with AKT2 antibody
AKT2 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 416-444 from the human protein was used as the immunogen for this AKT2 antibody.
Limitation:
This AKT2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
P31751