NPM1 Antibody

NSJ Bioreagents
Product Code: NSJ-F48247
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48247-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48247-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the NPM1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human lymph tissue stained with NPM1 antibody
2 / 4
Fluorescent image of A549 cell stained with NPM1 antibody. Alexa Fluor 488 secondary was used (green). NPM1 immunoreactivity is localized to the nucleus and nucleolus.
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NPM1 antibody western blot analysis in HeLa lysate.
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NPM1 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.

IHC analysis of FFPE human lymph tissue stained with NPM1 antibody
Fluorescent image of A549 cell stained with NPM1 antibody. Alexa Fluor 488 secondary was used (green). NPM1 immunoreactivity is localized to the nucleus and nucleolus.
NPM1 antibody western blot analysis in HeLa lysate.
NPM1 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the NPM1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nucleophosmin/NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 7-33 from the human protein was used as the immunogen for this NPM1 antibody.
Limitation:
This NPM1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
P06748