TFAM Antibody

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NSJ Bioreagents
Product Code: NSJ-F40574
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40574-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40574-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the TFAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from (1) HeLa, (2) HepG2 and (3) U-2OS cell line using TFAM antibody at 1:1000. Expected molecular weight: 24~29 kDa.
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TFAM antibody western blot analysis in (1) HeLa, (2) Jurkat, (3) K562, and (4) MCF-7 lysate; Expected molecular weight: 24~29 kDa.
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Western blot analysis of lysate from 293T cell line using TFAM antibody diluted at 1:1000. Expected molecular weight: 24~29 kDa.
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TFAM antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Fluorescent confocal image of NCI-H460 cell stained with TFAM antibody at 1:25. TFAM immunoreactivity is localized to mitochondrion.
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TFAM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma.
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Western blot analysis of lysate from (1) HeLa, (2) HepG2 and (3) U-2OS cell line using TFAM antibody at 1:1000. Expected molecular weight: 24~29 kDa.
TFAM antibody western blot analysis in (1) HeLa, (2) Jurkat, (3) K562, and (4) MCF-7 lysate; Expected molecular weight: 24~29 kDa.
Western blot analysis of lysate from 293T cell line using TFAM antibody diluted at 1:1000. Expected molecular weight: 24~29 kDa.
TFAM antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Fluorescent confocal image of NCI-H460 cell stained with TFAM antibody at 1:25. TFAM immunoreactivity is localized to mitochondrion.
TFAM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma.

Documents

Further Information

Application Details :
IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50,Western blot: 1:1000
Application Note:
Titration of the TFAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 216-246 from the human protein was used as the immunogen for this TFAM antibody.
Limitation:
This TFAM antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q00059