AKT1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F40742
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40742-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40742-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the AKT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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AKT1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma
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AKT1 antibody western blot analysis in MDA-MB435 lysate
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AKT1 antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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AKT1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma
AKT1 antibody western blot analysis in MDA-MB435 lysate
AKT1 antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 282-309 from the human protein was used as the immunogen for this AKT1 antibody.
Limitation:
This AKT1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Mouse, Rat, Xenopus
Uniprot #:
P31749