IL-12B Antibody

IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Western blot: 1:1000

Titration of the IL-12B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

IL12B is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Antigen affinity purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 264-290 from the human protein was used as the immunogen for this IL-12B antibody.

This IL-12B antibody is available for research use only.

Antigen affinity

Human

P29460