WNT5A Antibody

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NSJ Bioreagents
Product Code: NSJ-F49280
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49280-0.05ML0.05 ml£260.00
Quantity:
NSJ-F49280-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the WNT5A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC staining of FFPE human thyroid carcinoma with WNT5A antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human lung carcinoma with WNT5A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 5
Western blot testing of 1) human heart, 2) human PANC-1, 3) mouse heart and 4) rat heart lysate with WNT5A antibody. Expected molecular weight ~41 kDa.
4 / 5
Western blot testing of 1) human HeLa, 2) mouse brain, 3) mouse heart and 4) human PANC-1 lysate with WNT5A antibody. Expected molecular weight ~41 kDa.
5 / 5
WNT5A antibody flow cytometric analysis of HeLa cells (green) compared to a?negative control?(blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IHC staining of FFPE human thyroid carcinoma with WNT5A antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung carcinoma with WNT5A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) human heart, 2) human PANC-1, 3) mouse heart and 4) rat heart lysate with WNT5A antibody. Expected molecular weight ~41 kDa.
Western blot testing of 1) human HeLa, 2) mouse brain, 3) mouse heart and 4) human PANC-1 lysate with WNT5A antibody. Expected molecular weight ~41 kDa.
WNT5A antibody flow cytometric analysis of HeLa cells (green) compared to a?negative control?(blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the WNT5A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 185-213 from the human protein was used as the immunogen for this WNT5A antibody.
Limitation:
This WNT5A antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Rabbit
Uniprot #:
P41221