Anti-PCSK9 Antibody

NSJ Bioreagents
Product Code: NSJ-F50318
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50318-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50318-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the anti-PCSK9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot analysis of lysate from HeLa cell line using anti-PCSK9 antibody at 1:1000. Predicted size: Pro/mature ~74/64 kDa
2 / 5
Western blot analysis of lysate from HepG2 cell line using anti-PCSK9 antibody at 1:1000. Predicted size: Pro/mature ~74/64 kDa
3 / 5
IHC analysis of FFPE human colon carcinoma section using anti-PCSK9 antibody; Ab was diluted at 1:100.
4 / 5
Anti-PCSK9 antibody IHC analysis in formalin fixed and paraffin embedded human colon carcinoma.
5 / 5
Anti-PCSK9 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of lysate from HeLa cell line using anti-PCSK9 antibody at 1:1000. Predicted size: Pro/mature ~74/64 kDa
Western blot analysis of lysate from HepG2 cell line using anti-PCSK9 antibody at 1:1000. Predicted size: Pro/mature ~74/64 kDa
IHC analysis of FFPE human colon carcinoma section using anti-PCSK9 antibody; Ab was diluted at 1:100.
Anti-PCSK9 antibody IHC analysis in formalin fixed and paraffin embedded human colon carcinoma.
Anti-PCSK9 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:100,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting points. Titration of the anti-PCSK9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 479-508 from the human protein was used as the immunogen for this anti-PCSK9 antibody.
Limitation:
This anti-PCSK9 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q8NBP7