Alpha Actin Antibody

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NSJ Bioreagents
Product Code: NSJ-F44155
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44155-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44155-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the alpha Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Alpha Actin antibody western blot analysis in (1) A549, (2) RD, and (3) human placenta lysate
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Alpha Actin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle
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Confocal immunofluorescent analysis of alpha Actin antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Alpha Actin antibody flow cytometric analysis of CEM cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue).
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Alpha Actin antibody western blot analysis in (1) A549, (2) RD, and (3) human placenta lysate
Alpha Actin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle
Confocal immunofluorescent analysis of alpha Actin antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Alpha Actin antibody flow cytometric analysis of CEM cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the alpha Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with a-actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an a-actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 346-375 from human ACTA1 was used as the immunogen for this alpha Actin antibody.
Limitation:
This alpha Actin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Pig, Chicken, Rabbit, Xenopus
Uniprot #:
P68133