PAX6 Antibody

NSJ Bioreagents
Product Code: NSJ-F49967
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49967-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49967-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the PAX6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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PAX6 antibody western blot analysis in U251 lysate
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IHC analysis of FFPE human brain tissue stained with PAX6 antibody
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Confocal immunofluorescent analysis of PAX6 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
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Flow cytometric analysis of HeLa cells using PAX6 antibody (bottom histogram) compared to a negative control (top histogram).

PAX6 antibody western blot analysis in U251 lysate
IHC analysis of FFPE human brain tissue stained with PAX6 antibody
Confocal immunofluorescent analysis of PAX6 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Flow cytometric analysis of HeLa cells using PAX6 antibody (bottom histogram) compared to a negative control (top histogram).

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PAX6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 183-210 from the human protein was used as the immunogen for this PAX6 antibody.
Limitation:
This PAX6 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Xenopus
Uniprot #:
P26367