PCNA Antibody

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NSJ Bioreagents
Product Code: NSJ-F48249
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48249-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48249-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of PCNA antibody and Jurkat, HeLa, 293 lysate
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Western blot analysis of PCNA antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the PCNA gene.
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IHC analysis of FFPE human hepatocarcinoma tissue stained with PCNA antibody
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Confocal immunofluorescent analysis of PCNA antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Confocal immunofluorescent analysis of PCNA antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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PCNA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma.
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Western blot analysis of PCNA antibody and Jurkat, HeLa, 293 lysate
Western blot analysis of PCNA antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the PCNA gene.
IHC analysis of FFPE human hepatocarcinoma tissue stained with PCNA antibody
Confocal immunofluorescent analysis of PCNA antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of PCNA antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
PCNA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 231-261 from the human protein was used as the immunogen for this PCNA antibody.
Limitation:
This PCNA antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Rat, Bovine, Primate, Hamster
Uniprot #:
P12004