Anti-p53 Antibody
| Code | Size | Price |
|---|
| NSJ-F48067-0.08ML | 0.08 ml | £260.00 |
Quantity:
| NSJ-F48067-0.4ML | 0.4 ml | £502.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot the anti-p53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details :
IHC (Paraffin): 1:100,Western blot: 1:1000
Application Note:
Titration of the anti-p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 364-393 from the human protein was used as the immunogen for this anti-p53 antibody.
Limitation:
This anti-p53 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P04637