B-RAF Antibody

NSJ Bioreagents
Product Code: NSJ-F50793
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50793-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50793-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the B-RAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 2
Western blot analysis B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2). Predicted size 85-95 kDa~
2 / 2
IHC analysis of FFPE human brain tissue stained with B-RAF

Western blot analysis B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2). Predicted size 85-95 kDa~
IHC analysis of FFPE human brain tissue stained with B-RAF

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the B-RAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
B-RAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 379-408 from the human protein was used as the immunogen for this B-RAF antibody.
Limitation:
This B-RAF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
P15056