SPHK2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50220
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50220-0.05ML0.05 ml£260.00
Quantity:
NSJ-F50220-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SPHK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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SPHK2 antibody western blot analysis in HepG2 lysate.
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IHC analysis of FFPE human breast carcinoma tissue stained with the SPHK2 antibody
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IHC analysis of FFPE human kidney tissue stained with the SPHK2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 5
IHC analysis of FFPE human brain tissue stained with the SPHK2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of human 1) HepG2, 2) A549, 3) HEK293 and 4) U-87 MG lysate with SPHK2 antibody. Predicted molecular weight ~69 kDa.
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SPHK2 antibody western blot analysis in HepG2 lysate.
IHC analysis of FFPE human breast carcinoma tissue stained with the SPHK2 antibody
IHC analysis of FFPE human kidney tissue stained with the SPHK2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC analysis of FFPE human brain tissue stained with the SPHK2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) HepG2, 2) A549, 3) HEK293 and 4) U-87 MG lysate with SPHK2 antibody. Predicted molecular weight ~69 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the SPHK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 590-620 from the human protein was used as the immunogen for this SPHK2 antibody.
Limitation:
This SPHK2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q9NRA0