PHB Antibody

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NSJ Bioreagents
Product Code: NSJ-F48124
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48124-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48124-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PHB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human hepatocarcinoma tissue stained with PHB antibody
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Western blot testing of PHB antibody and HeLa cell lysate. Predicted molecular weight ~28 kDa.
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Confocal immunofluorescent analysis of PHB antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
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PHB antibody flow cytometric analysis of HeLa cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IHC analysis of FFPE human hepatocarcinoma tissue stained with PHB antibody
Western blot testing of PHB antibody and HeLa cell lysate. Predicted molecular weight ~28 kDa.
Confocal immunofluorescent analysis of PHB antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
PHB antibody flow cytometric analysis of HeLa cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
IHC (Paraffin): 1:10-1:50,Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PHB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 89-117 from the human protein was used as the immunogen for this PHB antibody.
Limitation:
This PHB antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Chicken
Uniprot #:
P35232