AKT1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50139
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50139-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50139-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the AKT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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AKT1 antibody western blot analysis in HeLa lysate
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Western blot analysis of AKT1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).
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IF testing of AKT1 antibody with MDA-MB435 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments stained red; DAPI nuclear counterstain (blue).
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IHC analysis of FFPE human breast carcinoma stained with AKT1 antibody
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AKT1 antibody flow cytometric analysis of MDA-MB435 cells (green) compared to a negative control (blue).
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AKT1 antibody western blot analysis in HeLa lysate
Western blot analysis of AKT1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).
IF testing of AKT1 antibody with MDA-MB435 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments stained red; DAPI nuclear counterstain (blue).
IHC analysis of FFPE human breast carcinoma stained with AKT1 antibody
AKT1 antibody flow cytometric analysis of MDA-MB435 cells (green) compared to a negative control (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. It and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 115-144 from the human protein was used as the immunogen for this AKT1 antibody.
Limitation:
This AKT1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Bovine
Uniprot #:
P31749