Phospho-ERBB2 Antibody (pT1172)

Immunofluorescence: 1:100,Dot blot: 1:500

Titration of the phospho-ERBB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Antigen affinity purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

This phospho-HER2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT1172 of human HER2/ERBB2.

This phospho-ERBB2 antibody is available for research use only.

Antigen affinity

Human

Mouse, Rat

P04626