Phospho-SMAD2 Antibody (pS118)
| Code | Size | Price |
|---|
| NSJ-F48605-0.08ML | 0.08 ml | £260.00 |
Quantity:
| NSJ-F48605-0.4ML | 0.4 ml | £502.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Dot Blot
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
Storage:
Aliquot the phospho-SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details :
Immunofluorescence: 1:10-1:50,Dot blot: 1:500
Application Note:
Titration of the phospho-SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-SMAD2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS118 of human SMAD2.
Limitation:
This phospho-SMAD2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Chicken, Drosophila, Mouse, Pig, Rat, Zebrafish
Uniprot #:
Q15796