Phospho-SMAD3 Antibody (pS208)
| Code | Size | Price |
|---|
| NSJ-F48449-0.08ML | 0.08 ml | £260.00 |
Quantity:
| NSJ-F48449-0.4ML | 0.4 ml | £502.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Dot Blot
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
Storage:
Aliquot the phospho-SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details :
Immunofluorescence: 1:10-1:100,Dot blot: 1:500
Application Note:
Titration of the phospho-SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-SMAD3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS208 of human SMAD3.
Limitation:
This phospho-SMAD3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Pig, Chicken
Uniprot #:
P84022