Phospho-ULK1 Antibody (pS556)

NSJ Bioreagents
Product Code: NSJ-F48759
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48759-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48759-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
Storage:
Aliquot the phospho-ULK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 2
Fluorescent image of Chloroquine treated U251 cells stained with phospho-ULK1 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.~
2 / 2
Dot blot analysis of phospho-ULK1 antibody. 50ng of phos-peptide o

Fluorescent image of Chloroquine treated U251 cells stained with phospho-ULK1 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.~
Dot blot analysis of phospho-ULK1 antibody. 50ng of phos-peptide o

Further Information

Application Details :
Immunofluorescence: 1:200,Dot blot: 1:500
Application Note:
Titration of the phospho-ULK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells (By similarity). [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-ULK1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS556 of human ULK1.
Limitation:
This phospho-ULK1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
O75385