p-NANOG Antibody (pS71)

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NSJ Bioreagents
Product Code: NSJ-F48601
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48601-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48601-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
Storage:
Aliquot the p-NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Fluorescent confocal image of SY5Y cells stained with p-NANOG antibody at 1:50. Immunoreactivity is localized very specifically to the nuclei.~
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Dot blot analysis of p-NANOG antibody. 50ng of phos-peptide or nonphos-peptide per dot were
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Fluorescent confocal image of SY5Y cells stained with p-NANOG antibody at 1:50. Immunoreactivity is localized very specifically to the nuclei.~
Dot blot analysis of p-NANOG antibody. 50ng of phos-peptide or nonphos-peptide per dot were

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Dot blot: 1:500
Application Note:
Titration of the p-NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This p-NANOG antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS71 of human Nanog.
Limitation:
This p-NANOG antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Primate
Uniprot #:
Q9H9S0