p-EGFR Antibody (pY1069)

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NSJ Bioreagents
Product Code: NSJ-F48475
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48475-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48475-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot the p-EGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of EGFR in HeLa cell lysate, either noninduced (Lane 1) or induced with EGF (2).
2 / 3
Western blot analysis of extracts from A431 cells,untreated or treated with EGF, using p-EGFR antibody (left) or nonphos Ab (right).
3 / 3
Dot blot analysis of p-EGFR antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted. P-Peptide=phos-peptide; NP-Peptide=nonphos-peptide.
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Western blot analysis of EGFR in HeLa cell lysate, either noninduced (Lane 1) or induced with EGF (2).
Western blot analysis of extracts from A431 cells,untreated or treated with EGF, using p-EGFR antibody (left) or nonphos Ab (right).
Dot blot analysis of p-EGFR antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted. P-Peptide=phos-peptide; NP-Peptide=nonphos-peptide.

Documents

Further Information

Application Details :
Dot blot: 1:500,Western blot: 1:1000
Application Note:
Titration of the p-EGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. EGFR is a receptor for EGF, but also for other members of the EGF family, including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This p-EGFR antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1069 of human EGFR.
Limitation:
This p-EGFR antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P00533