Phospho-HER3 Antibody (pY1289)

Dot blot: 1:500,Western blot: 1:1000

Titration of the phospho-HER3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form.

Antigen affinity purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

This phospho-HER3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1289 of human HER3/ERBB3.

This phospho-HER3 antibody is available for research use only.

Antigen affinity

Human

P21860