p-p21 Antibody (pT145)
| Code | Size | Price |
|---|
| NSJ-F48424-0.08ML | 0.08 ml | £260.00 |
Quantity:
| NSJ-F48424-0.4ML | 0.4 ml | £502.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Dot Blot
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot the p-p21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details :
Western blot: 1:1000,Dot blot: 1:500,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the p-p21 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
P21CIP1 is a potent cyclin-dependent kinase inhibitor. This protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21CIP1 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein has been reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This p-p21 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT145 of human P21CIP1.
Limitation:
This p-p21 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P38936