Histone H4 Antibody (Methyl-Lys20 specific)

NSJ Bioreagents
Product Code: NSJ-F48612
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48612-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48612-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the H4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot analysis of lysate from K562 cell line using H4 antibody diluted at 1:1000 for each lane.
2 / 4
IHC analysis of FFPE human lung carcinoma stained with H4 antibody
3 / 4
H4 antibody flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
Dot blot analysis of H4 antibody (Methyl 2/methylation-specific). 50ng of methylation-peptide or Non methylation-peptide per dot were spotted.

Western blot analysis of lysate from K562 cell line using H4 antibody diluted at 1:1000 for each lane.
IHC analysis of FFPE human lung carcinoma stained with H4 antibody
H4 antibody flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Dot blot analysis of H4 antibody (Methyl 2/methylation-specific). 50ng of methylation-peptide or Non methylation-peptide per dot were spotted.

Further Information

Application Details :
Western blot: 1:1000,Dot blot: 1:500,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Amino acids surrounding the K20 methylation site of the human protein were used as the immunogen for this Histone H4 antibody.
Limitation:
This H4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P62805