Histone H3.3 Antibody (H3F3A)

NSJ Bioreagents
Product Code: NSJ-F40711
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40711-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40711-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot the Histone H3.3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Dot blot analysis of phospho-Histone H3.3 antibody. 50ng of Methyl-peptide or Non Methyl-peptide per dot were spotted.

Dot blot analysis of phospho-Histone H3.3 antibody. 50ng of Methyl-peptide or Non Methyl-peptide per dot were spotted.

Further Information

Application Details :
Dot blot: 1:500
Application Note:
Titration of the Histone H3.3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from human H3F3A was used as the immunogen for this Histone H3.3 antibody.
Limitation:
This Histone H3.3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P84243