Phospho-AKT2 Antibody (pS474)

NSJ Bioreagents
Product Code: NSJ-F48486
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48486-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48486-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot the phospho-AKT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Dot blot analysis of phospho-AKT2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted. P-Pab=phos-Ab; P-Peptide=phos-peptide; NP-Peptide=nonphos-peptide.

Dot blot analysis of phospho-AKT2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted. P-Pab=phos-Ab; P-Peptide=phos-peptide; NP-Peptide=nonphos-peptide.

Further Information

Application Details :
Dot blot: 1:500
Application Note:
Titration of the phospho-AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-AKT2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS474 of human AKT2.
Limitation:
This phospho-AKT2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P31751