Phospho-BRAF Antibody (pT400)

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NSJ Bioreagents
Product Code: NSJ-F48555
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48555-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48555-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot the phospho-BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Dot blot analysis of phospho-BRAF antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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Dot blot analysis of phospho-BRAF antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

Documents

Further Information

Application Details :
Dot blot: 1:500
Application Note:
Titration of the phospho-BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), and a wide range of cancers such as lung cancer, non-Hodgkins lymphoma, and colorectal cancer.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-BRAF antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT400 of human BRAF.
Limitation:
This phospho-BRAF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
P15056