Phospho-ERBB2 Antibody (pY1005)

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NSJ Bioreagents
Product Code: NSJ-F48698
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48698-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48698-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the phospho-ERBB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of extracts from A431 cells, untreated or treated with EGF, using phospho-ERBB2 antibody (left) or nonphos Ab (right).
2 / 3
Fluorescent confocal image of MCF7 cells stained with phospho-ERBB2 antibody at 1:100. Note the highly specific localization to the plasma membrane and cytoplasm.
3 / 3
Dot blot analysis of phospho-ERBB2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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Western blot analysis of extracts from A431 cells, untreated or treated with EGF, using phospho-ERBB2 antibody (left) or nonphos Ab (right).
Fluorescent confocal image of MCF7 cells stained with phospho-ERBB2 antibody at 1:100. Note the highly specific localization to the plasma membrane and cytoplasm.
Dot blot analysis of phospho-ERBB2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:100,Dot blot: 1:500
Application Note:
Titration of the phospho-ERBB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-ERBB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1005 of human ERBB2.
Limitation:
This phospho-ERBB2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P04626