Phospho-SMAD4 Antibody (pT277)

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NSJ Bioreagents
Product Code: NSJ-F48451
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48451-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48451-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Storage:
Aliquot the phospho-SMAD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of phospho-SMAD4 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
2 / 3
Dot blot analysis of phospho-SMAD4 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
3 / 3
IHC analysis of FFPE breast carcinoma stained with phospho-SMAD4 antibody.
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Confocal immunofluorescent analysis of phospho-SMAD4 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Dot blot analysis of phospho-SMAD4 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
IHC analysis of FFPE breast carcinoma stained with phospho-SMAD4 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Dot blot: 1:500,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the phospho-SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-SMAD4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT277 of human SMAD4.
Limitation:
This phospho-SMAD4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
Q13485