HIF1 alpha Antibody

NSJ Bioreagents
Product Code: NSJ-F48865
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48865-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48865-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Hamster
  • Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the HIF1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of HIF1 alpha antibody and CHO lysate
2 / 3
HIF1 alpha antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma.
3 / 3
Fluorescent confocal image of HeLa cell stained with HIF1 alpha antibody at 1:25. HIF1A immunoreactivity is localized to the cytoplasm and nucleus.

Western blot analysis of HIF1 alpha antibody and CHO lysate
HIF1 alpha antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma.
Fluorescent confocal image of HeLa cell stained with HIF1 alpha antibody at 1:25. HIF1A immunoreactivity is localized to the cytoplasm and nucleus.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This protein encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for this HIF1 alpha antibody.
Limitation:
This HIF1 alpha antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Hamster
Species Reactivity (Predicted):
Bovine
Uniprot #:
Q16665