Shh Antibody / Sonic Hedgehog

NSJ Bioreagents
Product Code: NSJ-F53068
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F53068-0.08ML0.08 ml£260.00
Quantity:
NSJ-F53068-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the Shh antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from mouse liver tissue lysate using Shh antibody. Predicted molecular weight: 45/27/19 kDa (1)~
2 / 2
Flow cytometric analysis of HeLa cells using Shh antibody (green) compared to an <a href=../search_result.

Western blot analysis of lysate from mouse liver tissue lysate using Shh antibody. Predicted molecular weight: 45/27/19 kDa (1)~
Flow cytometric analysis of HeLa cells using Shh antibody (green) compared to an <a href=../search_result.

Further Information

Application Details :
Flow Cytometry: 1:25,Western blot: 1:1000 (1)
Application Note:
Titration of the Shh antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The 45 kDa precursor protein autocleaves into 27 kDa amino and 19 kDa carboxy fragments.

Description:
Sonic hedgehog binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 395-450 were used as immunogen for this Shh antibody.
Limitation:
This Shh antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q62226