SMAD2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50345
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50345-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50345-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:100. Note the highly specific localization of the SMAD2 to the nucleus.
2 / 3
SMAD2 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 3
Western blot analysis of SMAD2 antibody and NIH3T3 lysate. Predicted molecular weight: 52~60 kDa.
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Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:100. Note the highly specific localization of the SMAD2 to the nucleus.
SMAD2 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of SMAD2 antibody and NIH3T3 lysate. Predicted molecular weight: 52~60 kDa.

Documents

Further Information

Application Details :
Immunofluorescence: 1:100,Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 97-125 from the human protein was used as the immunogen for this SMAD2 antibody.
Limitation:
This SMAD2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Bovine, Pig, Chicken, Zebrafish, Drosophila
Uniprot #:
Q15796