EGR1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F48309
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48309-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48309-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the EGR1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Confocal immunofluorescent analysis of EGR1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
2 / 4
EGR1 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 4
Western blot analysis of EGR1 antibody and mouse NIH3T3 lysate. Predicted molecular weight ~58 kDa.
4 / 4
EGR1 antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~58 kDa.
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Confocal immunofluorescent analysis of EGR1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
EGR1 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of EGR1 antibody and mouse NIH3T3 lysate. Predicted molecular weight ~58 kDa.
EGR1 antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~58 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the EGR1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
EGR1 belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 9-37 from the human protein was used as the immunogen for this EGR1 antibody.
Limitation:
This EGR1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Bovine, Xenopus
Uniprot #:
P18146