ILK2/ILK1 Antibody

Western blot: 1:1000

Titration of the ILK2/ILK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) is an ankyrin repeat containing 51 kDa receptor-proximate serine-threonine kinase (1), with a reported migration rate of 59K. This 451 amino acid protein interacts with the cytoplasmic domain of the beta-1 integrin subunit and contains sequence motifs found in pleckstrin homology domains capable of interacting with phosphoinositide lipids. ILK is an upstream regulator of Pi(3)K dependant activation of protein kinase B (PKB/AKT) and inhibition of glycogen synthase kinase 3 (GSK-3). ILK2 expression is associated with mediation of cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. ILK2 is overexpressed in some highly invasive tumor cell lines.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 391-421 from the human protein was used as the immunogen for this ILK2/ILK1 antibody.

This ILK2/ILK1 antibody is available for research use only.

Purified

Human, Mouse

Rat, Bovine

Q13418