mGLUR5 Antibody

Western blot: 1:1000

Titration of the mGLUR5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 (also known as GPRC1E) and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. The activity of GRM5 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 1020-1050 from the human protein was used as the immunogen for this mGLUR5 antibody.

This mGLUR5 antibody is available for research use only.

Purified

Human, Mouse

P41594